The integration of usability evaluations into iterative software development processes is becoming increasingly relevant, as gathering feedback from prospective users is crucial to improving the usability of eHealth systems. Traditional approaches to eHealth usability evaluation, however, are difficult to reconcile with the iterative software development process, which is why there is an increasing need for agile usability evaluation. Despite the existence of approaches to agile usability evaluation, it is unclear whether they are suitable to rapidly evaluate patient-centered eHealth systems being iteratively developed in health care applicable, for prospective users such as elderly persons suffering from age-related declines. This research includes an innovative and comprehensive toolbox called ToUsE, toolbox for eHealth usability evaluation, which consists of more than 40 rapidly deployable and potentially useful eHealth usability evaluation methods developed by the author. The ToUsEtoolbox is intended for use by software developers, medical informaticians, usability experts, or medical professionals who aim at performing an eHealth usability evaluation. The book provides detailed descriptions of all the eHealth usability evaluation methods included in ToUsE.