This volume presents a collection of computational and experimental prools pertaining to the creation, characterization, and utilization of RNA nanostructures. The chapters in this book cover topics such as ion effects in RNA folding; design and crystallography of self-assembling RNA nanostructures; x-aptamer selection and validation; RNAi in HIV-infected cells; and preparation of a conditional RNA switch. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough,
RNA Nanostructures: Methods and Prools
is a valuable resource for the design and production of RNA nanostructures. Researchers and scientists sharing these detailed prools is important for sustained progress in the field.